Biodiversity Data Analysis

In the previous two experiments, students set out to measure the biodiversity of two communities in Chattanooga: Signal Point Park and the Confederate Cemetery. This week, students aimed to analyze the data of the whole class that was collected during this time and compare the biodiversity of the two communities. To do this, students used the Shannon Diversity Index to calculate the observed biodiversity value (H’), the maximum diversity value possible (Hmax), the total species richness (S), and species evenness (J). When compared to Signal Point Park, the cemetery will have a greater Shannon Diversity Index value and thus greater biodiversity due to the greater human impact on the Signal Point Park community. 

Signal Point Park is a community nestled on Signal Mountain in Chattanooga, Tennessee. The area sampled by students was a field adjacent to the parking lot, and a walking path ran through it to connect the lot to hiking trails and the mountain overlook. The Confederate Cemetery, on the other hand, is closer to the campus of the University of Tennessee at Chattanooga, nestled just across from Holt Hall. It is a cemetery, so it is maintained and visited. The occasional student may walk through the community to get to class, too. 

Students used the same methods to sample biodiversity from both communities. Students broke into four groups and obtained a 1m2 quadrat. They generated a random number and walked forward that many steps. With a second random number, they walked to the right and placed the quadrat at their feet. Students recorded a description of each species found within the quadrat, and they recorded the number of individuals within each species. They repeated this until they sampled a total of ten areas. With four student groups sampling ten plots each, that came to a total of 40 areas sampled for each community. Back in the lab, students added up the number of individuals found within all their quadrants.

In order to calculate the Shannon Diversity Index values of the communities, students used the following equations:

P=  (Number of individuals of one species)/(total number of individuals)

H’ = ∑ [-p (ln p)]

S= total number of species

Hmax= lnS

J= H’/Hmax

From these equations, students calculated that Community A (Signal Point Park) had an H’ value of 2.54 and an Hmax value of 3.61. It had 37 total species and a species evenness of 0.70. These values are listed in Table 1. In Community B (Confederate Cemetery), the H’ value was determined to be 2.48 and the Hmax value was 3.58. It had 36 total species and a species evenness of 0.69. The determination of these values is listed in Table 2.

Table 1. Species Abundance and Shannon Diversity Index Values of Signal Point Park

Species Abundance Proportion -p(ln p)
Granddaddy Long Leg Spider 1 0.00070 0.0051
Jumping Spider 2 0.0014 0.0091
Beetle 1 0.00070 0.0051
Moss 14 0.0097 0.045
Black Ants 56 0.039 0.13
Red Ants 3 0.0021 0.013
Small Red Insects 2 0.0014 0.0091
Lichen 67 0.047 0.14
Sawtoothed plant 45 0.031 0.11
Clover 83 0.058 0.16
Pine 2 0.0014 0.0091
Red Bud 6 0.0047 0.023
Wild Onion 47 0.033 0.11
Poison Oak 1 0.00070 0.0051
Heart-shaped Leaf Grass 14 0.0097 0.045
Wild Strawberry 51 0.035 0.12
Small, Woody Shrub 18 0.013 0.055
Pointed Shrub 1 0.00070 0.0051
Broad-leaf plant 99 0.069 0.18
Dandelion 17 0.012 0.052
Tear-shaped Plant 12 0.0083 0.040
Maple 1 0.00070 0.0051
Violet 20 0.014 0.059
Yellow Violet Vine 31 0.022 0.083
Flowering Vine 18 0.013 0.055
Thin Grass 304 0.21 0.33
Broad Grass 401 0.28 0.36
Short, fuzzy plant 16 0.011 0.050
Stemmed Grass 10 0.0070 0.035
Bunny Grass 7 0.0049 0.026
3 squiggly leafed plant 1 0.00070 00.051
Oval leaf sapling 3 0.0021 0.013
Branched leaf plant 11 0.0076 0.037
Prickly grass 16 0.011 0.050
Bumpy grass 41 0.029 0.101
Broad diamond leaf plant 8 0.0056 0.029
Lobed grass 8 0.0056 0.029
Total Individuals 1438 H’ 2.54
Total Number of Species 37
J 0.70
Hmax 3.61

Table 2. Species Abundance and Shannon Diversity Index Values of the Confederate Cemetery

Species Abundance Proportion -p(ln p)
Lichen 96 0.034 0.12
Wild Strawberry 7 0.0025 0.015
Pillbug 7 0.0025 0.015
Red bug 1 0.00036 0.0028
Leaf Bug 2 0.00071 0.0052
Weavel 1 0.00036 0.0028
Snails 5 0.0018 0.011
Spider 2 0.0071 0.0052
Fruit Fly 1 0.00036 0.0028
Caterpillar 1 0.00036 0.0028
Black Ants 476 0.17 0.30
Red Ants 1 0.00036 0.0028
Clover 144 0.051 0.15
Moss 10 0.0036 0.020
Poison Oak 4 0.0014 0.0093
Vines 12 0.0043 0.023
Two-leaf Vine 55 0.020 0.077
Ivy 5 0.0018 0.011
Lily pad leaves 141 0.050 0.15
Thin bladed grass 763 0.27 0.35
Broad bladed grass 192 0.068 0.18
Violets 80 0.029 0.10
Three leaf sapling 36 0.013 0.056
Broad sapling 23 0.0082 0.039
Sawtooth leaves 87 0.031 0.11
Woody stem plant 138 0.049 0.15
Long grass 121 0.043 0.14
Small leafy branch 15 0.0053 0.028
Flower shaped leaves 2 0.00071 0.0052
Fuzzy leaf 3 0.0011 0.0073
Oval leaf 3 0.0011 0.0073
Rounded leaf 16 0.0057 0.029
Ruffled leaf 42 0.015 0.063
Heart-shaped grass 281 0.10 0.23
Lobed grass 24 0.0086 0.041
Stemmed grass 10 0.0036 0.020
Total Individuals 2807 H’ 2.48
Total Number of Species 36
J 0.69
Hmax 3.58

The relative abundances of each species in both communities is graphed as a rank abundance curve in Figure 1.

Figure 1. The Rank Abundance Curves of Signal Point Park and the Confederate Cemetery

The blue line plots the rank abundance of species in Signal Point Park. The orange line plots the rank abundance of species in the Confederate Cemetery.

The Signal Point Park community has a higher H’ value. Thus, it has higher biodiversity than the Confederate Cemetery. Both communities have a relatively moderate diversity when compared to their Hmax values. My hypothesis is disproved by the data. Signal Point Park has the greater biodiversity when calculated with the class data set. When calculated with only my group’s data, however, this was not the case. The Confederate Cemetery was found to have an H’ value of 1.92, which is higher than the H’ value of 1.87 calculated for Signal Point Park. The differences in biodiversity between the two communities is minimal, and it is likely that human impact is roughly equal in both areas. Additionally, there could have been an error in recording data from either community. One group may have had a different name for the same species, causing the total species richness to be counted higher than it actually is. Lastly, when comparing the two communities in Figure 1, students can see that both communities are dominated by a few species. Overall, though, they have highly similar species evenness

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